Please use this identifier to cite or link to this item: https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/908
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dc.contributor.authorVulcu, Felicia-
dc.contributor.authorLeong, Vivian-
dc.contributor.authorMullarkey, Caitlin-
dc.contributor.otherGauthier, Taylor-
dc.contributor.otherLeong, Clark-
dc.date.accessioned2021-07-26T21:09:03Z-
dc.date.available2021-07-26T21:09:03Z-
dc.date.issued2021-07-
dc.identifier5b44ae2e-f1ff-4331-846a-52cf93d60eb9-
dc.identifier.urihttps://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/908-
dc.description.tableofcontents1. An introduction to lab basics and your research project-
dc.description.tableofcontents2. Restriction enzyme digestion reactions and agarose gel electrophoresis-
dc.description.tableofcontents3. PCR amplicon purification, DNA ligation, and transformation-
dc.description.tableofcontents4. Plasmid DNA purification: minipreparations (aka. alkaline lysis minipreps)-
dc.description.tableofcontents5. Plasmid DNA mapping using restriction enzymes-
dc.description.tableofcontents6. DHFR protein expression, cell lysis and buffer preparation-
dc.description.tableofcontents7. DHFR protein purification using Ni-NTA affinity chromatography-
dc.description.tableofcontents8. DHFR protein quantitation (the Bradford assay)-
dc.description.tableofcontents9. Protein visualization: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)-
dc.description.tableofcontents10. Crystallizing and visualizing DHFR protein crystals-
dc.description.tableofcontents11. Bacterial Cell Inhibition Assay-
dc.language.isoengen_US
dc.publisherDepartment of Biochemistry and Biomedical Sciences (undergraduate program), Faculty of Health Sciences, McMaster Universityen_US
dc.publisherDepartment of Biochemistry and Biomedical Sciences (undergraduate program), Faculty of Health Sciences, McMaster University-
dc.relation.isformatofhttps://ecampusontario.pressbooks.pub/biochem2l06/-
dc.rightsCC BY-NC | https://creativecommons.org/licenses/by-nc/4.0/en_US
dc.titleBBS OER Lab Manualen_US
dc.typeLearning objecten_US
dcterms.accessRightsOpen Accessen_US
dcterms.educationLevelUniversity - Undergraduate-
dc.identifier.slughttps://openlibrary.ecampusontario.ca/catalogue/item/?id=5b44ae2e-f1ff-4331-846a-52cf93d60eb9-
dc.rights.holderFelicia Vulcu/ Vivian Leong/ Caitlin Mullarkey (Department of Biochemistry and Biomedical Sciences McMaster University)en_US
ecO-OER.AdoptedNoen_US
ecO-OER.AncillaryMaterialNoen_US
ecO-OER.InstitutionalAffiliationMcMaster Universityen_US
ecO-OER.ISNI0000 0004 1936 8227en_US
ecO-OER.ReviewedNoen_US
ecO-OER.PODNo-
ecO-OER.AccessibilityStatementUnknown-
lrmi.learningResourceTypeInstructional Object - Teaching/Learning Strategyen_US
lrmi.learningResourceTypeLearning Resource - Reference Materialen_US
ecO-OER.POD.compatibleYesen_US
dc.description.abstractThe Biochemistry and Biomedical Sciences (BBS) OER is a laboratory manual for an undergraduate fundamental Biochemistry laboratory course. The BBS OER lab manual features custom videos of laboratory techniques, interactive content, and elevated instructor resources. The lab manual consists of eleven laboratory experiments immersing students in a directed research project centered on the overarching theme of drug discovery. We also developed an "instructor resources" chapter. This chapter highlights our unique approach to lab course delivery.en_US
dc.subject.otherSciences - Biochemistry & Biomedical Scienceen_US
ecO-OER.ItemTypeInteractive Material-
ecO-OER.MediaFormateBook-
ecO-OER.MediaFormatHTML/XML-
ecO-OER.MediaFormatOffice applications-
ecO-OER.MediaFormatPDF-
Appears in Collections:Ontario OER Collection

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