BBS OER Lab Manual

Vulcu, Felicia; Leong, Vivian; Mullarkey, Caitlin

dc.contributor.author	Vulcu, Felicia
dc.contributor.author	Leong, Vivian
dc.contributor.author	Mullarkey, Caitlin
dc.contributor.other	Gauthier, Taylor
dc.contributor.other	Leong, Clark
dc.date.accessioned	2021-07-26T21:09:03Z
dc.date.available	2021-07-26T21:09:03Z
dc.date.issued	2021-07
dc.identifier	5b44ae2e-f1ff-4331-846a-52cf93d60eb9
dc.identifier.uri	https://openlibrary-repo.ecampusontario.ca/jspui/handle/123456789/908
dc.description.tableofcontents	1. An introduction to lab basics and your research project
dc.description.tableofcontents	2. Restriction enzyme digestion reactions and agarose gel electrophoresis
dc.description.tableofcontents	3. PCR amplicon purification, DNA ligation, and transformation
dc.description.tableofcontents	4. Plasmid DNA purification: minipreparations (aka. alkaline lysis minipreps)
dc.description.tableofcontents	5. Plasmid DNA mapping using restriction enzymes
dc.description.tableofcontents	6. DHFR protein expression, cell lysis and buffer preparation
dc.description.tableofcontents	7. DHFR protein purification using Ni-NTA affinity chromatography
dc.description.tableofcontents	8. DHFR protein quantitation (the Bradford assay)
dc.description.tableofcontents	9. Protein visualization: Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE)
dc.description.tableofcontents	10. Crystallizing and visualizing DHFR protein crystals
dc.description.tableofcontents	11. Bacterial Cell Inhibition Assay
dc.language.iso	eng	en_US
dc.publisher	Department of Biochemistry and Biomedical Sciences (undergraduate program), Faculty of Health Sciences, McMaster University	en_US
dc.publisher	Department of Biochemistry and Biomedical Sciences (undergraduate program), Faculty of Health Sciences, McMaster University
dc.relation.isformatof	https://ecampusontario.pressbooks.pub/biochem2l06/
dc.rights	CC BY-NC \| https://creativecommons.org/licenses/by-nc/4.0/	en_US
dc.title	BBS OER Lab Manual	en_US
dc.type	Learning object	en_US
dcterms.accessRights	Open Access	en_US
dcterms.educationLevel	University - Undergraduate
dc.identifier.slug	https://openlibrary.ecampusontario.ca/catalogue/item/?id=5b44ae2e-f1ff-4331-846a-52cf93d60eb9
dc.rights.holder	Felicia Vulcu/ Vivian Leong/ Caitlin Mullarkey (Department of Biochemistry and Biomedical Sciences McMaster University)	en_US
ecO-OER.Adopted	No	en_US
ecO-OER.AncillaryMaterial	No	en_US
ecO-OER.InstitutionalAffiliation	McMaster University	en_US
ecO-OER.ISNI	0000 0004 1936 8227	en_US
ecO-OER.Reviewed	No	en_US
ecO-OER.POD	No
ecO-OER.AccessibilityStatement	Unknown
lrmi.learningResourceType	Instructional Object - Teaching/Learning Strategy	en_US
lrmi.learningResourceType	Learning Resource - Reference Material	en_US
ecO-OER.POD.compatible	Yes	en_US
dc.description.abstract	The Biochemistry and Biomedical Sciences (BBS) OER is a laboratory manual for an undergraduate fundamental Biochemistry laboratory course. The BBS OER lab manual features custom videos of laboratory techniques, interactive content, and elevated instructor resources. The lab manual consists of eleven laboratory experiments immersing students in a directed research project centered on the overarching theme of drug discovery. We also developed an "instructor resources" chapter. This chapter highlights our unique approach to lab course delivery.	en_US
dc.subject.other	Sciences - Biochemistry & Biomedical Science	en_US
ecO-OER.ItemType	Interactive Material
ecO-OER.MediaFormat	eBook
ecO-OER.MediaFormat	HTML/XML
ecO-OER.MediaFormat	Office applications
ecO-OER.MediaFormat	PDF